Fig. 5.

Drp1 silencing attenuates CPT-induced mitochondrial fragmentation and apoptosis. a The effects of CPT on mitochondrial fusion/fission proteins expression in OS cells. Representative immunoblot of the protein levels of Drp1, Opa1, Mfn1 and Mfn2. b Comparison of mitochondrial morphology in the control and Drp1 siRNA cells. Images of mitochondria (red) and nucleus (blue) were collected by confocal microscope. c Drp1 silencing reversed the reduction of cell viability in CPT treated 143B cells. Representative dot plots of Annexin V/PI analysis after CPT treatments in the presence or absence of Drp1 siRNA. d CPT decreased ATP production and increased ADP/ATP ratio of both 143B and MG63 cells. The results were expressed as the means ± SD from three independent experiments. *P < 0.05, significantly different compared with control