Figure 3.

Isolation and Characterization of Different Preparations of Exosomes

(A) Schema of the procedure to isolate exosomes from MSC-conditioned media. Exosomes were collected in chemically defined protein-free media during 48 h and then were concentrated and purified by using anion exchange chromatography. The elution fractions containing exosomes were pooled and dialyzed against PBS. (B) Characterization of exosomes by nanoparticle tracking analysis (NTA). Representative size distribution profiles of the different exosome preparations used in this study. See Table 1 for concentration (particles per milliliter) and size (in nanometers) of exosome samples according to NTA measures.