Figure 2.

PHF5A expression is drastically upregulated in HCC cell lines and fresh HCC tissues. (a) PHF5A expression is significantly increased in HCC cell lines compared with immortalized normal liver cell LO2 by qPCR (left panel) and western blotting (right panel) assay. (b) qPCR (left panel) and western blotting (right panel) assay showed that PHF5A expression is dramatically upregulated in fresh HCC tissues compared with ANT. (c) IHC assay demonstrated that PHF5A is markedly increased in HCC tissues compared with ANT using paired tissue slides. ANT: corresponding adjacent normal tissues.