(a).
| Accession number | Gene | Primer type | Primer sequences (5′–3′) |
|---|---|---|---|
| NM_001289746 | GAPDH | Forward | TTGCCCTCAACGACCACTTT |
| Reverse | TGGTCCAGGGGTCTTACTCC | ||
|
| |||
| NM_004864.2 | GDF15 | Forward | CAGGACGGTGAATGGCTCTC |
| Reverse | TAGCGTTTCCGCAACTCTCG | ||
|
| |||
| NM_001432.2 | EREG | Forward | CTGCAGGTGTGAAGTGGGTT |
| Reverse | GTGGAACCGACGACTGTGAT | ||
|
| |||
| NM_015713.4 | RRM2B | Forward | AGAGTTCTCGCCGGTTTGTC |
| Reverse | GTCGACCTCTTCTGCTGTCC | ||
|
| |||
| NM_016848.5 | SHC3 | Forward | GCTTTTGCTGTGAGCAGCC |
| Reverse | CCACCGTTAAAAGCCAGCAC | ||
|
| |||
| NM_183001.4 | SHC1 | Forward | CTTGGGAGCTACATTGCCTGT |
| Reverse | TCAAAAAGCTCTCTGCCTGGAC | ||
|
| |||
| NM_014454.2 | SESN1 | Forward | TTGACAGCTCCACAACGTGA |
| Reverse | TGTACACGAAAGGGCAGTCT | ||
|
| |||
| NM_005259.2 | MSTN | Forward | TTGACATGAACCCAGGCACT |
| Reverse | GTCCTGGGAAGGTTACAGCA | ||
|
| |||
| NM_002478.4 | MYOD1 | Forward | CGCCAGGATATGGAGCTACT |
| Reverse | GAGTGCTCTTCGGGTTTCAG | ||
|
| |||
| NM_005902.3 | SMAD3 | Forward | CATGTCGTCCATCCTGCCTT |
| Reverse | TTGGTGTTGACGTTCTGCGT | ||