(b).
| Cycling step | Temperature | Time | Number of cycles |
|---|---|---|---|
| Enzyme activation/initial DNA denaturation | 95°C | 30 seconds | 1 |
| Denaturation | 95°C | 5 seconds | 40 |
| Annealing/extension | 60°C | 30 seconds | |
| Melt curve | 65°C to 95°C (in 0.5°C increments) | 5 seconds | 1 |
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