Figure 4.

Effect of scaffold type on hMSSM-derived cells-mediated transcription of different osteoblastic markers. Cells were seeded either alone or on collagen, gelatin, or HA/βTCP/Fibrin scaffold and cultivated in osteogenic differentiation medium for 14 days. Quantitative real-time PCR was used to assess the mRNA levels of Runx2 (Panel (a)), ON (Panel (b)), OCN (Panel (c)), OPN (Panel (d)), BMP-2 (Panel (e)), and COL1 (Panel (f)). Data were normalized to GAPDH levels. Each value represents a mean ± SEM for three independent experiments (n=3) each done in triplicate. ∗p<0.05; ∗∗p<0.01, ∗∗∗p<0.001 vs. cells with gelatin scaffold (Student's t-test).