Figure 3.

QKF alleviate neuronal apoptosis in the hippocampus of APP/PS1 mice.

Notes: (A) TUNEL staining in the hippocampal CA1 area of each group of mice (original magnification, ×400). (B) Statistical analysis results of TUNEL staining. (C) Protein expression of Bax, Bcl-2, and Caspase-3 in the hippocampus. (D) ODs indicative of Bax protein expression. (E) ODs indicative of Bcl-2 protein expression. (F) ODs indicative of caspase-3 protein expression. OD values of Bax, Bcl-2, and caspase-3 expression in each group were quantified and analyzed from the WB results. (G) Levels of cleaved caspase-3 in mice brain were determined by an ELISA assay. Values are expressed as mean±SEM, n=5 per group. Significant differences between two groups are indicated as **P<0.01. M-QKF, middle-dose QKF group.

Abbreviations: QKF, qingxin kaiqiao fang; WB, Western blot; SEM, standard error of the mean.