Visualization of γδ T cells and mAbs penetration within the MALC. (a,c,e,g) Temporal evolution of relative area of mAbs (A, E) and γδ T cells (C, G) with respect to the MALC total area (0 = no penetration, 1 = full penetration) (A, C, n = 3; E, G n = 4). (b,d,f,h) Dynamics and spatial distribution pattern of penetration of mAbs (B, F) and γδ Tcells (D, H) (blue = low intensity signal, yellow = high intensity signal) within the MALC for one experiment. (i) Visualization of MALC-GFP (green), GA101 (purple) and γδ T cells (red) at multiple time points. All the images were taken from the same z-plane inside the MALC.