(A) Western blotting of overexpressed Fkbp5 mutants and Fkbp4 in Fkbp5−/− myoblasts.
(B) EdU uptake in WT and Fkbp5−/− myoblasts transduced with the Fkbp genes before (day 0) and during differentiation (day 1).
(C) EdU uptake in WT and Fkbp5−/− myoblasts transduced with the Fkbp5 genes on differentiation day 1.
(D) Immunostaining of MHC in WT and Fkbp5−/− myoblasts transduced with the Fkbp5 genes on differentiation day 1.
(E) The differentiation index in WT and Fkbp5−/− myoblasts transduced with the Fkbp genes before (day 0) and during differentiation (day 1).
**p < 0.01 with Student’s t test in comparison to the empty vector. Data are presented as mean + SD of technical triplicates.