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. Author manuscript; available in PMC: 2019 Jan 29.
Published in final edited form as: Cell Rep. 2018 Nov 27;25(9):2537–2551.e8. doi: 10.1016/j.celrep.2018.11.006

Figure 7. Requirement of Cdk4 P173 for the Inhibition of C2C12 Cell Proliferation by Fkbp5.

Figure 7.

(A) Immunostaining of overexpressed rCdk4-WT and rCdk4-P173A in Cdk4 KD cells. Scale bar, 100 μm.

(B-E) rCdk4-WT and rCdk4-P173A were transduced into C2C12 cells after double KD of Fkbp5 and Cdk4. The cells were used to analyze EdU uptake (B), the differentiation index (C), the 1-nucleus index (D), and qPCR of muscle genes (E). **p < 0.01 with Student’s t test. Data are presented as mean + SD of technical triplicates.