Table II.
Comparison of basic and functional semen parameters in different classes of 25-OHD concentration
| 25-OHD<10 ng ml -1 (n= 26) | 10 ng ml -1 ≤25-OHD<20 ng ml -1 (n= 31) | 25-OHD≥20 ng ml -1 (n= 5) | |
|---|---|---|---|
| Sperm concentration (M/ml) | 17.4 ± 3.9 | 23.55 ± 3.8 | 26.4 ± 8.5 |
| Sperm total motility (%) | 29.54 ± 3.5 | 35.84 ± 2.7 | 38.4 ± 5.7 |
| Normal morphology (%) | 3.34 ± 0.7 | 5.54 ± 1 | 5.2 ± 1.6 |
| 25-OHD concentration (ng/ml) | 7.53 ± 0.3 | 15.07 ± 0.5a | 36.8 ± 7.7b |
| TUNEL positive cells (%) | 14.37 ± 1.6 | 9.82 ± 1.2 | 9.63 ± 2.1 |
| ROS content (RLU/min) | 0.79 ± 0.09 | 0.57 ± 0.09 | 0.42 ± 0.18 |
| hMMP sperms (%) | 50.76 ± 3.4 | 59.9 ± 4.8 | 70.23 ± 0.96 |
Data were presented as mean±standard error of mean (Kruskal-Wallis test followed by Dunn’s multiple comparison, and ANOVA followed by Tukey’s multiple comparison)
25-OHD: 25-hydroxy vitamin D
ROS: reactive oxygen species
hMMP: high mitochondrial membrane potential
a
; significant difference between insufficient and deficient groups
b
; significant difference between sufficient and deficient groups