Table III.
Comparison of basic and functional semen parameters in NS and OAT men categorized according to concentration of 25-OHD
|
25-OHD<10 ng ml
-1
|
10 ng ml
-1
≤25-OHD<20 ng ml
-1
|
25-OHD≥20 ng ml
-1
|
||||
|---|---|---|---|---|---|---|
| NS (n= 5) | OAT (n= 21) | NS (n= 12) | OAT (n= 19) | NS (n= 3) | OAT (n= 2) | |
| Sperm concentration (M/ml) | 42.8 ± 17.3 | 11.36 ± 0.6 | 43.4 ± 6.6 | 11 ± 0.8 | 36.67 ± 10.5 | 11 ± 3 |
| Sperm total motility (%) | 55.6 ± 8 | 23.33 ± 2.5 | 50.75 ± 2.2 | 26.42 ± 2.4 | 47.33 ± 1.4 | 25 ± 5 |
| Normal morphology (%) | 10 ± 1.6 | 1.76 ± 0.2 | 11.58 ± 1.4 | 1.73 ± 0.26 | 7.66 ± 1.2 | 1.5 ± 0.5 |
| 25-OHD concentration (ng/ml) | 6.94 ± 0.8 | 7.68 ± 0.3 | 14.63 ± 0.7a | 15.34 ± 0.7a | 33.87 ± 8.4a | 41.2 ± 18.9a |
| TUNEL positive cells (%) | 14.09 ± 4.4 | 14.44 ± 1.7 | 7.01 ± 1.3 | 11.61 ± 1.6 | 7.82 ± 3.2 | 12.35 ± 1.3 |
| ROS content (RLU/min) | 0.45 ± 0.15 | 0.86 ± 0.11 | 0.24 ± 0.04 | 0.78 ± 0.12 | 0.34 ± 0.2 | 0.54 ± 0.43 |
| hMMP sperms (%) | 65.91 ± 5.5 | 48.24 ± 3.4 | 68.24 ± 4.07 | 50.53 ± 8.3 | 70.5 ± 1.6 | 69.7 ± 2.8 |
Data were presented as mean ± standard error of mean (Kruskal–Wallis test followed by Dunn’s multiple comparison)
25-OHD: 25-hydroxy vitamin D
NS: normozoospermic
OAT: oligoasthenoteratozoospermic
ROS: reactive oxygen species
hMMP: High mitochondrial membrane potential
a
; significant difference when compared with its counterpart in deficient group (25-OHD<10 ng ml-1)