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. Author manuscript; available in PMC: 2019 Jan 29.
Published in final edited form as: Immunohorizons. 2018 Jul 23;2(5):155–163. doi: 10.4049/immunohorizons.1700062

FIGURE 1. Reduction of FRC number in aged spleens.

FIGURE 1.

Spleens were mechanically and enzymatically digested to liberate stromal cells for flow cytometric quantification. (A) Total number of nucleated cells in young and aged spleens. (B) Samples were gated on live single cells that were CD45− (left). FRCs (PDPN+CD31−), BECs (PDPN−CD31+), and DNCs (PDPN− CD31−) were further gated as shown (right). (C) Numbers of CD45− stromal cells, (D) FRCs, (E) BECs, and (F) DNCs in young and aged spleens. Data are pooled from three independent experiments (n = 7–8 mice per group). Error bars = ±SEM. Statistical significance was determined by two-tailed t test. *p < 0.05.