KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-HA | Sigma-Aldrich | Cat# H3663; RRID:AB_262051 |
| Rabbit polyclonal anti-Protein A | Sigma-Aldrich | Cat# P3775; RRID:AB_261038 |
| Mouse monoclonal anti-phospho-Threonine-Proline | Cell Signaling | Cat# 9391; RRID:AB_331801 |
| Mouse monoclonal anti-GST | Sigma-Aldrich | Cat# G7781; RRID:AB_259965 |
| Rabbit polyclonal anti-TbPSA6 | Li et al., 2002 | N/A |
| Rabbit polyclonal anti-eIF4E4 | Gift by O.P. de Melo Neto | N/A |
| Rabbit polyclonal anti-eIF4G3 | Gift by O.P. de Melo Neto | N/A |
| Rat monoclonal YL1/2 | EMD Millipore | Cat# MAB1864; RRID:AB_2210391 |
| IRDye 680LT anti-rabbit IgG | Li-Cor | 925-68022 |
| FITC-conjugated anti-rat IgG | Sigma-Aldrich | Cat# F6258; RRID:AB_259695 |
| FITC-conjugated anti-mouse IgG | Sigma-Aldrich | Cat# F0257; RRID:AB_259378 |
| Critical Commercial Assays | ||
| Phos-tag | Wako Pure Chemical, Inc | 300-93523 |
| Click-iT EdU Alexa Fluor 488 imaging kit | ThermoFisher | C10337 |
| Poly(A)-agarose | Jena Bioscience | AC-159S |
| γ-Aminophenyl-m7GTP (C10-spacer)-Agarose | Jena Bioscience | AC-155 |
| lambda protein phosphatase | New England Biolabs | P0753 |
| RNase A | Sigma-Aldrich | R6513 |
| IgG Sepharose 6 Fast Flow affinity resin | GE Healthcare | 17-0969-01 |
| EZview anti-HA affinity gel | Sigma-Aldrich | Cat# E6779; RRID:AB_10109562 |
| Complete Mini EDTA-free protease inhibitor cocktail | Roche | 11836170001 |
| QuickChange Site-directed mutagenesis kit | Agilent Technologies | 200524 |
| Glutathione-Sepharose 4B beads | GE Healthcare | 17075601 |
| Experimental Models: Cell lines | ||
| Trypanosoma brucei 29-13 cell line | ATCC | PRA-381 |
| Trypanosoma brucei Lister427 | ATCC | NR-42010 |
| Oligonucleotides | ||
| Extensive primer sequences provided in Table S1 | This study | N/A |
| Deposited data | ||
| Raw mass spectrometry data for eIF4E4 and PABP1 phosphosite identification: The PeptideAtlas repository, accession number: PASS01291 | This study | https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/PASS_View?identifier=PASS01291 |
| Original dataset: Mendeley database | This study | https://data.mendeley.com/datasets/xwk46s3vg7/1 |
| Recombinant DNA | ||
| pC-eIF4E4-PTP-NEO | This study | N/A |
| pC-PABP1-PTP-NEO | This study | N/A |
| pC-PABP1-3HA-PAC | This study | N/A |
| pSL-PABP1-BLE | This study | N/A |
| pSL-PABP1-3′UTR-PAC | This study | N/A |
| pLew100-eIF4E4-PTP-BLE | This study | N/A |
| pLew100-eIF4E4-S102A-PTP-BLE | This study | N/A |
| pLew100-eIF4E4-S102E-PTP-BLE | This study | N/A |
| pLew100-eIF4E4-7A-PTP-BLE | This study | N/A |
| pLew100-eIF4E4-7E-PTP-BLE | This study | N/A |
| pLew100-eIF4E4-3HA-BLE | This study | N/A |
| pLew100-eIF4E4-7A-3HA-BLE | This study | N/A |
| pLew100-eIF4E4-7E-3HA-BLE | This study | N/A |
| pLew100-PABP1-3HA-BLE | This study | N/A |
| pLew100-PABP1-3A-3HA-BLE | This study | N/A |
| pLew100-PABP1-3E-3HA-BLE | This study | N/A |
| pGEX-4T-3-CYC2 | Hu et al., 2016 | N/A |
| pCDF-CRK1 | Hu et al., 2016 | N/A |
| pGEX-4T-3-eIF4E4 | This study | N/A |
| pGEX-4T-3-eIF4E4-7A | This study | N/A |
| pGEX-4T-3-PABP1 | This study | N/A |
| pGEX-4T-3-PABP1-3A | This study | N/A |