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. 2019 Jan 29;14(1):e0211268. doi: 10.1371/journal.pone.0211268

Table 1. Summary of the parameters/conditions used in the primary HTS assay.

Cell line	PANC-1	A549
Cell suspension volume	25 μl/well in medium
Cell number	2000 cells/well
PCL compounds/controls	30 nl in DMSO
C_tot (PCL compounds)	10 μM, 0.1% DMSO
V (Pt drugs/PBS/water)	5 μl/well in medium
C_tot (cisplatin) Ctrl/IC₂₀/IC₅₀	0/5/15 μM	0/1.5/7 μM
C_tot (carboplatin) Ctrl/IC₂₀/IC₅₀	0/20/100 μM	0/15/90 μM
C_tot (oxaliplatin) Ctrl/IC₂₀/IC₅₀	0/2/16 μM	0/0.5/2.5 μM
Drugs exposure time	72 h	50 h
V (PrestoBlue)	3 μl/well, 1 h incubation time