. 2019 Jan 29;14(1):e0211211. doi: 10.1371/journal.pone.0211211

Table 1. Matrix of the 2² CCRD.

Coded and real levels of aeration and agitation and the response variables of the R. solanacearum RS inoculum incubated for 24 h at 32°C in YM culture medium, using sucrose as the carbon source.

Treataments	Independent variables			Dependent variables
Treataments	Aeration (vvm)	Agitation (rpm)	pH	OD_600nm	DCW (g.L^-1)	P(3HB) (%)	Productivity from the DCW (g.L^-1.h^-1)
1	-1 (0.3)	-1 (150)	6.3	8.61	2.13	26.5	0.08
2	+1 (1)	-1 (150)	5.7	15.31	4.0	39.65	0.15
3	-1 (0.3)	+1 (250)	5.8	15.37	4.81	36.62	0.19
4	+1 (1)	+1 (250)	5.9	18.04	4.82	42.87	0.19
5	-1.41 (0.25)	0 (200)	6.3	11.73	2.00	23.11	0.07
6	+1.41 (1.05)	0 (200)	6.5	13.24	1.93	45.06	0.06
7	0 (0.65)	-1.41 (143)	6.3	10.82	2.85	43.98	0.10
8	0 (0.65)	+1.41 (257)	5.8	13.99	4.33	39.94	0.17
9	0 (0.65)	0 (200)	5.7	16.09	4.51	31.76	0.17
10	0 (0.65)	0 (200)	5.7	16.40	4.50	31.17	0.17
11	0 (0.65)	0 (200)	5.7	15.44	4.57	31.28	0.18

pH, potential of hydrogen; OD_600nm, optical density; DCW, dry cell weight; P(3HB), poly(3-hydroxybutyrate).

Table 1. Matrix of the 22 CCRD.

Table 1. Matrix of the 2² CCRD.