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. Author manuscript; available in PMC: 2019 Jan 29.
Published in final edited form as: Cell Rep. 2018 Oct 9;25(2):464–477.e4. doi: 10.1016/j.celrep.2018.09.042

Figure 4. Binucleated I-Cells and S-Cells Are Likely to Increase Ploidy by Endocycling.

Figure 4.

(A) A section from the urothelium of an untreated adult mouse stained for expression of Ecad and EdU. Scale bar, 50 µm.

(B) A section from an adult mouse 48 hr after CPP, given a 1-hr pulse of EdU stained for EdU and Ecad. Note EdU-expressing binucleated I-cells, which are likely to be in S phase. Scale bar, 50 µm.

(C) A section from an adult mouse 72 hr after CPP, given a 1-hr pulse of EdU prior to sacrifice stained for expression of Ecad and EdU. Note EdU-expressing S-cells, which are likely to be in S phase. Scale bar, 50 µm.

(D) Same sample used in (A) but at a higher magnification.

(E) Same sample used in (B) but at a higher magnification.

(F) Same sample used in (C) but at a higher magnification.

(G) A paraffin stained section from an adult mouse 24 hr p.i. analyzed 1 hr after a pulse of EdU and stained for EdU and P63 expression. Scale bar, 25 µm.

(H) A paraffin-stained section from an adult mouse 72 hr p.i. analyzed 1 hr after a pulse of EdU and stained for EdU and P63 expression. Scale bar, 25 µm.

(I) A table showing a summary of quantification of binucleated I-cells and S-cells in cytokinesis.

(J) A bar graph showing the percentage of EdU+ binucleated I-cells and S-cells after a 1-hr pulse, which are likely to be in S phase, in animals 24–72 hr after UTI and CPP-induced injury and repair.