KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| p63 (4A4) antibody | Santa Cruz Biotechnology | SC8431: RRID:AB 628091 |
| p63 antibody [N2C1), Internal | GenTex | GTX102425: RRIDiAB 1952344 |
| Keratin 5 Polyclonal Antibody | Covance | PRB-160P: RRID:AB 10063444 |
| Keratin 5 Polyclonal Chicken Antibody | Covance | SIG-3475; RRID:AB 10720202 |
| Cytokeratin 20 antibody | DAKO | M7019: RRIDiAB 2133718 |
| Ki67 antibody - Proliferation Marker | Abeam | ab15580: RRIDiAB 443209 |
| Green Fluorescent Protein (GFP) Antibody | AVES Lab | GFP-1020. RRIDiAB 10000240 |
| UPIII (M-17) antibody | Santa Cruz Biotechnology | sc-15186: RRIDAB 671435 |
| Survivin (71G4B7) Rabbit mAb antibody | Cell signaling technology | 2808S: RRIDiAB 10691694 |
| Mouse monoclonal Anti-beta-Tubulin antibody | Sigma | T4026: RRIDiAB 477577 |
| Phospho-Histone H3 (Seri 0) (6G3) Mouse monoclonal Antibody | Cell signaling technology | 9706S; RRIDiAB 331748 |
| Rabbit Anti-Cyclin D1 Monoclonal Antibody | Abeam | ab16663: RRIDiAB 443423 |
| Goat Anti-Mouse E-cadherin Polyclonal antibody.Unconjugated | R&D system | AF748; RRIDiAB 355568 |
| Anti-RFP (RABBIT) Polyclonal Antibody | Rockland | 600–401-379; RRIDiAB 2209751 |
| Aurora A/B kinase. Mouse monoclonal | EnCor Biotechnology | MCA-5A12: RRIDiAB 2572231 |
| Anillin Antibody, Rabbit Polyclonal | Nov us | NBP1–31060; RRIDiAB 2058287 |
| Alexa Fluor® 488-AffiniPure Donkey Anti-Rabbit IgG (H+L) antibody | Jackson Immuno-Research Labs | 711–545-152: RRIDiAB 2313584 |
| Alexa Fluor 488-AffiniPure Donkey Anti-Mouse IgG (H+L) antibody | Jackson Immuno-Research Labs | 715–545-150: RRIDiAB 2340846 |
| Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) antibody | Jackson Immuno-Research Labs | 711–165-152; RRIDiAB 2307443 |
| Alexa Fluor 594-AffiniPure Donkey Anti-Mouse IgG (H+L) antibody | Jackson Immuno-Research Labs | 715–585-151; RRIDiAB 2340855 |
| Alexa Fluor 647-AffiniPure Donkey Anti-Mouse IgG (H+L) antibody | Jackson Immuno-Research Labs | 715–605-150; RRIDiAB 2340862 |
| Alexa Fluor 647-AffiniPure Donkey Anti-Rabbit IgG (H+L) antibody | Jackson Immuno-Research Labs | 711–605-152: RRIDiAB 2492288 |
| Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY(lgG) (H+L) antibody | Jackson Immuno-Research Labs | 703–545-155; RRIDiAB 2340375 |
| Alexa Fluor 594-AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) antibody | Jackson Immuno-Research Labs | 703–585-155; RRIDiAB 2340377 |
| Bacterial and Virus Strains | ||
| Uropathogenic E.coi Strain UTI 89 | Gift from Scott Hultgren Lab | N/A |
| Biological Samples | ||
| Mouse adult female bladder. UTI infected and control animals. | This paper | N/A |
| Mouse urothelium: dissected tissue for the organotypic culture. | This paper | N/A |
| Mouse urothelium. CPP-treated male/female. | This Paper | N/A |
| Chemicals. Peptides, and Recombinant Proteins | ||
| EdU (ô-ethynyl-^-deoxyuridine). pulse chase | Invitrogen | Cat# A10044 |
| Tamoxifen, for inducing Cre recombinase | Sigma | cat# T5648 |
| 4-OHT. for urothelial culture | Sigma | #H7904 |
| CPP for injury/repair model | Sigma | cat #C7397 |
| KSFM and supplements for tissue culture | GIBCO | #17005042 |
| Critical Commercial Assays | ||
| Click-iT EdU Alexa Fluor 647 Imaging Kit | Invitrogen | Cat #C 10340 |
| Experimental Models: Organisms/Strains | ||
| Mouse line: FVB.Cg-Tg(KRT5-cre/ERT2) 2lpc/JeldJ | Obtained from D. Metzger and P. Cham bon (Indra et al., 1999) |
RRIDJMSR JAX:018394 |
| Mouse line: Upk2CreERT (B6:CBA-Tg (Upk2-icre/ERT2)1 Ccc) | Obtained from the Cord on-Card o lab. (Shen et al.. 2012) |
RRIDJMSR JAX:024768 |
| Mouse line Upk3aGCE (B6:DBA-Tg (Upk3a-GFP/cre/ERT2)26Amc/J | Obtained from the McMahon lab | RRIDJMSR JAX:015855 |
| Mouse line B6;129S6-Gt(ROSA) 26Sortm14(CAG-tdTomato)Hze/J, (Rosa26:mCheriy) | The Jackson Laboratory | RRIDJMSR JAX:007908 |
| Mouse line Rosa26mTmG *B6.129(Cg) Gt(ROSA)26Sortm4(ACT– tdTomato,–EGFP) Luo/J33) | The Jackson Laboratory (Muzumdar et al.. 2007) | RRIDJMSR JAX:007676 |
| Oligonucleotides | ||
| Forward Upk2CreER: CATTGCCCCTGTTTCACTATC | This paper | N/A |
| Reverse Upk2CreER CAATGATGTTTCACTGGTTATG | This paper | N/A |
| Forward Krt5CreER ATTTGCCTGCATTACCGGTC | This paper | N/A |
| Reverse Krt5CreER ATCAACGTTTTGTTTTCGGA | This paper | N/A |
| Forward R26mGmT/R26nTnG CTCTGCTGCCTC CTGGCTTCT | This paper | N/A |
| Reverse R26mGmT/R26nTnG TCAATGGGCGG GGGTCGTT | This paper | N/A |
| Forward Upk3aGCE AGGAGCTTTGCAGAAGACCA | This paper | N/A |
| Reverse Upk3aGCE GAACTTCAGGGTCAGCTTGC | This papier | N/A |
| Forward Rosa26:mCherry GGCATTAAAGCAGC GTATCC | This paper | N/A |
| Reverse Rosa26:mCherry CTGTTCCTGTACGG CATGG | This paper | N/A |
| Software and Algorithms | ||
| NIS-Elements | Nikon | RRID:SCR_014329; https://www.nikon.com/products/microscopesolutions/support/download/software/imgsfw/nis_v41100mac.htm |
| Fiji package ImageJ | ImageJ | https://fiji.se/ |
| Prism | GraphPad | PRISM, RRID:SCR_005375 RRID:SCR_000306; https://www.graphpad.com/scientificsoftware/prism/ |
| Other | ||
| Zeiss Axiovert 200M microscope with an Apotome | Zeiss | N/A |
| Nikon Eclipse TE200 microscope | Nikon | N/A |
| Confocal microscopy. A1R MP confocal microscope | Nikon | N/A |