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. 2019 Jan 17;8:e43788. doi: 10.7554/eLife.43788

Figure 5. FBF-2 variants retain base recognition specificity at flanking positions.

Figure 5.

Yeast 3-hybrid analyses of binding by PUF-8, FBF-2 SS/Y, FBF-2 AS/Y, and FBF-2 AQ/Y to an MS2 hairpin fused to 8-nt PBE RNAs bearing nucleotide substitutions at positions 3–5. Binding activity is shown as units of β-galactosidase activity normalized to cell count. Error bars indicate the standard deviation of three replicate measurements. Source data areavailable in Figure 5—source data 1.

Figure 5—source data 1. Raw data for Beta-Glo assay of FBF-2, FBF-2 R5 variants and PUF-8 that carry R2 SE/H mutations with different length binding elements.
elife-43788-fig5-data1.xlsx (10.9KB, xlsx)
DOI: 10.7554/eLife.43788.018