Figure 6. The FBF-2 R5 AQ/Y variant partially rescues the tumorous phenotype in the C.elegans germ line caused by loss of PUF-8 in a sensitized genetic background.

Extruded germlines were stained for nuclei (DAPI, blue) or mitotic cells (α-PHH3, red). (A) Fluorescence microscopic image of an extruded germline from an animal with wild-type fbf-2 and a gain-of-function mutation (glp-1(ar202)) that was subjected to puf-8 depletion by RNAi. These animals produce tumors throughout the germline as evidenced by the presence of red mitotic cells. (B) Fluorescence microscopic image of an extruded germline from an edited animal with FBF-2 AQ/Y variant (fbf-2(lot14)) and a gain-of-function mutation (glp-1(gf)) that was subjected to puf-8 depletion by RNAi. Note the absence of red mitotic cells throughout the gonad.