Table 1. Data collection and refinement statistics.
| Protein:RNA | PUF-8: PBE |
FBF-2 SS/Y: PBE |
FBF-2 AS/Y: PBE |
FBF-2 AQ/Y: PBE | |
|---|---|---|---|---|---|
| Data collection | |||||
| Space group | C2 | P61 | P61 | P61 | |
| Unit Cell |
a, b, c (Å) |
109.2, 189.0, 63.2 |
96.4, 96.4, 99.9 |
96.5, 96.5, 101.1 |
95.9, 95.9, 100.4 |
| α, β, γ (°) |
90, 103.6, 90 |
90, 90, 120 |
90, 90, 120 |
90, 90, 120 |
|
| Resolution (Å) | 50–2.55 (2.59–2.55)* |
50–2.25 (2.29–2.25)* |
50–2.25 (2.33–2.25)* |
50–2.85 (2.9–2.85)* |
|
| Rsym or Rmerge | 0.191 (0.692) | 0.101 (0.704) | 0.104 (0.772) | 0.191 (0.957) | |
| I /σI | 9.4 (1.9) | 19.2 (3.42) | 17.1 (2.98) | 12.8 (2.34) | |
| Completeness (%) | 98.9 (98.0) | 99.9 (100) | 99.9 (100) | 99.6 (99.2) | |
| Redundancy | 6.9 (3.6) | 5.7 (5.7) | 5.7 (5.7) | 10.7 (8.8) | |
| Refinement | |||||
| Resolution (Å) | 33.8–2.6 | 32.0–2.3 | 31.6–2.3 | 27.5–2.9 | |
| No. reflections | 37,625 | 25,089 | 25,386 | 12,185 | |
| Rwork/Rfree | 0.229/ 0.285 |
0.158/ 0.204 |
0.167/ 0.223 |
0.219/ 0.272 |
|
| No. atoms | |||||
| Protein | 8415 | 3197 | 3194 | 3189 | |
| RNA | 507 | 150 | 168 | 168 | |
| Solvent | 229 | 169 | 109 | 21 | |
| B-factors (Å2) | |||||
| Wilson B | 29.7 | 36.6 | 35.5 | 48.4 | |
| Protein | 32.8 | 45.3 | 45.1 | 50.2 | |
| RNA | 43.6 | 51.4 | 58.8 | 64.8 | |
| Solvent | 32.7 | 49.6 | 44.3 | 23.1 | |
| R.m.s deviations | |||||
| Bond lengths (Å) | 0.002 | 0.007 | 0.007 | 0.002 | |
| Bond angles (°) | 0.45 | 0.77 | 0.78 | 0.38 | |
*Values in parentheses are for the highest-resolution shell.