Table 2. RNA-binding analyses of PUF-8 and FBF-2 proteins.
Table 2—source data 1. Data for Table 2.
elife-43788-table2-data1.xlsx (9.5KB, xlsx)
DOI: 10.7554/eLife.43788.006
| Protein | RNA | RNA sequence | Kd (nM) | Krel* |
|---|---|---|---|---|
| PUF-8 | PBE | UGUA UAUA | 28.8 ± 0.7 | 1 |
| PBE-A5 | UGUA AAUA | 25.9 ± 3.1 | 0.9 | |
| PBE-C5 | UGUA CAUA | 44.8 ± 2.4 | 1.6 | |
| PBE-G5 | UGUA GAUA | 45.6 ± 3.1 | 1.6 | |
| FBE | UGUGCCAUA | 3110 ± 656 | 108 | |
| FBF-2 WT | PBE | ACAUGUAA AUAC | 74.6 ± 7.7 | 1 |
| FBE | ACAUGUGCCAUAC | 19.3 ± 0.6 | 0.3 | |
| FBF-2 SS/Y | PBE | ACAUGUAA AUAC | 22.7 ± 0.4 | 1 |
| FBE | ACAUGUGCCAUAC | 50.9 ± 2.3 | 2.2 | |
| FBF-2 AS/Y | PBE | ACAUGUAA AUAC | 16.3 ± 0.8 | 1 |
| FBE | ACAUGUGCCAUAC | 76.7 ± 4.3 | 4.7 | |
| FBF-2 SS/R | PBE | ACAUGUAA AUAC | 51.1 ± 1.1 | 1 |
| FBE | ACAUGUGCCAUAC | 26.9 ± 2.3 | 0.5 | |
| FBF-2 AQ/Y | PBE | ACAUGUAA AUAC | 20.2 ± 1.2 | 1 |
| FBE | ACAUGUGCCAUAC | 79.9 ± 2.0 | 4.0 |
*Krel values are calculated for each protein with binding to the PBE RNA set to 1.