Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 21;7:e35478. doi: 10.7554/eLife.35478

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

PMC Copyright notice

Figure 3. — (A) The nuclear RNAi pathway silences somatic targets. dsRNA targeting the lir-1 mRNA leads to the generation of 22G small RNAs in the cytoplasm and the nuclear RNAi pathway uses these 22G RNAs to target the endogenous lir-1/let-26 locus for silencing. (B) nhl-2(ok818) worms are resistant to lir-1 RNAi. This phenotype was rescued in transgenic nhl-2(ok818) worms expressing an GFP-NHL-2 extrachromosomal array (Ex+) under control of the putative nhl-2 promoter. Percent larval arrest represents mean of two biological replicates ±SD; **=P values <0.001, *=P values <0.0418, n > 100. (C) nhl-2(ok818) worms display a mortal germline phenotype at 25°C. Error bars indicate mean ±SD, n = 6. (D) Analysis of transgenerational brood size and embryonic lethality (E) of wild-type and nhl-2(ok818) worms. ****=P values <0.0001, ***=P values <0.0004, *=P values <0.0418. Error bars indicate mean ±SD, n = 37. (F) nhl-2(ok818) worms have a minor defect in RNAi inheritance at 20°C. Transgenic worms of the indicated genotypes expressing pie-1::gfp::h2b were fed gfp RNAi for one generation and then grown on OP50 thereafter. Animals were scored for GFP expression using a fluorescence microscope at 63X objective. The percentage of animals expressing GFP is shown, n ≥ 37. Germlines of nhl-2(ok818) worms grown at 25°C were unable to be scored due to the disorganised nature of the proximal gonad.

Figure 3—figure supplement 1. — (A) The nuclear RNAi pathway silences somatic targets. dsRNA targeting the lir-1 mRNA leads to the generation of 22G small RNAs in the cytoplasm and the nuclear RNAi pathway uses these 22G RNAs to target the endogenous lir-1/let-26 locus for silencing. (B) nhl-2(ok818) worms are resistant to lir-1 RNAi. This phenotype was rescued in transgenic nhl-2(ok818) worms expressing an GFP-NHL-2 extrachromosomal array (Ex+) under control of the putative nhl-2 promoter. Percent larval arrest represents mean of two biological replicates ±SD; **=P values <0.001, *=P values <0.0418, n > 100. (C) nhl-2(ok818) worms display a mortal germline phenotype at 25°C. Error bars indicate mean ±SD, n = 6. (D) Analysis of transgenerational brood size and embryonic lethality (E) of wild-type and nhl-2(ok818) worms. ****=P values <0.0001, ***=P values <0.0004, *=P values <0.0418. Error bars indicate mean ±SD, n = 37. (F) nhl-2(ok818) worms have a minor defect in RNAi inheritance at 20°C. Transgenic worms of the indicated genotypes expressing pie-1::gfp::h2b were fed gfp RNAi for one generation and then grown on OP50 thereafter. Animals were scored for GFP expression using a fluorescence microscope at 63X objective. The percentage of animals expressing GFP is shown, n ≥ 37. Germlines of nhl-2(ok818) worms grown at 25°C were unable to be scored due to the disorganised nature of the proximal gonad.