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GcAVs contain PtdIns4P. (a) HeLa cells expressing EmGFP-OSBP-PH were infected with GAS for 4 h and immunostained with anti-TGOLN2 antibody. (b) HeLa cells expressing EmGFP-OSBP-PH and mCherry-LC3 were infected with GAS for 4 h. (c) HeLa cells expressing mCherry-LC3 were infected with GAS for 4 h and immunostained with anti-PtdIns4P antibody. Bars: 10 μm. NI, noninfected.
