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. 2018 Sep 25;15(3):453–465. doi: 10.1080/15548627.2018.1520548

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 3. — Autophagic flux was impaired in CERKL-depleted ARPE-19 cells. (a and b) Immunoblotting and quantification of the levels of endogenous LC3-II:LC3-I ratio in negative control (NC) and CERKL-depleted ARPE-19 cells under DMSO and 2.5 nM bafilomycin A₁ condition for 3 h. Means± SEM of 5 repeats are shown. (c) Immunostaining analysis of the colocalization GFP-LC3 and RFP-LAMP1 in NC and CERKL-depleted ARPE-19 cells. Scale bars: 10 μm.