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. 2018 Sep 25;15(3):453–465. doi: 10.1080/15548627.2018.1520548

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 7. — CERKL regulated the stability of SIRT1. (a) SIRT1 protein levels at indicated times post treatment with 200 μg/ml cycloheximide (CHX) in negative control (NC) and CERKL-depleted ARPE-19 cells. (b) Quantification of protein presented in panel A. Relative expression of SIRT1 in relation to GAPDH. Means± SEM of 3 repeats are shown. (c and d) Immunostaining and quantification of the protein levels of SIRT1 p-S27 and p-S47 in NC and CERKL-depleted ARPE-19 cells. Relative expression of proteins in relation to TUBA. Means± SEM of 5 repeats are shown.