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. 2018 Sep 25;15(3):453–465. doi: 10.1080/15548627.2018.1520548

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 9. — CERKL directly interacted with SIRT1. (a and b) Reciprocal co-immunoprecipitation assays. ARPE-19 cell extracts transfected with plasmids encoding GFP-CERKL and FLAG-SIRT1 were immunoprecipitated with one indicated tag antibody (FLAG in A, and GFP in B) and analyzed by immunoblotting analysis with the other antibody. (c) Immunostaining analysis of the colocalization of CERKL and SIRT1 in ARPE-19 cells transfected with plasmids encoding GFP-CERKL (green) and FLAG-SIRT1 (red). Scale bars: 10 μm. (d) ARPE-19 cell extracts from cells transfected with plasmids encoding GFP-CERKL and FLAG-SIRT1 were immunoprecipitated with GFP antibody and analyzed by immunoblotting analysis with FLAG antibody and SIRT1 p-S27 antibody. (e) ARPE-19 cell extracts from cells transfected with plasmids encoding GFP-CERKL and wild-type or mutant forms of FLAG-SIRT1 were immunoprecipitated with GFP and analyzed by immunoblotting analysis with FLAG antibody.