Table 1.
Table List of primary antibodies used in this study.
| Antibodies | Source | Recognize | Dilution |
|---|---|---|---|
| Anti-LC3B | Abcam Ab48394 | Zebrafish and human LC3B | 1:1000 for WB 1:200 for IF |
| Anti-CERKL | Sigma-Aldrich HPA035444 | Human CERKL | 1:1000 for WB |
| Anti-SQSTM1/p62 | ABclonal A7758 | Human SQSTM1 | 1:1000 for WB |
| Anti-ATG5 | ABclonal A7252 | Human ATG5 | 1:1000 for WB |
| Anti-ATG7 | ABclonal A0691 | Human ATG7 | 1:1000 for WB |
| Anti-SIRT1 | ABclonal A11267 | Zebrafish and human SIRT1 | 1:700 for WB |
| Proteintech 13161-1-AP | Zebrafish and human SIRT1 | 1:700 for WB | |
| Anti-phospho-SIRT1 (Ser27) | Cell Signaling Technology 2327 | Human SIRT1 p-S27 | 1:500 for WB |
| Anti-phospho-SIRT1 (Ser47) | Cell Signaling Technology 2314 | Human SIRT1 p-S47 | 1:200 for WB |
| Anti-pan acetyl-lysine | ABclonal A2391 | 1 mg per 1 ml cell lysate for IP | |
| Anti-AMPK | ABclonal A1229 | Human AMPKα | 1:1000 for WB |
| Anti-AMPK p-T172 | ABclonal AP0116 | Human phospho-PRKAA/AMPKα p-T172 | 1:1000 for WB |
| Anti-BECN1/BECLIN1 | Proteintech 11306-1-AP | Human BECN1 | 1:700 for WB |
| Anti-TUBA/α-TUBULIN | Proteintech 11224-1-AP | Zebrafish and human TUBA | 1:5000 for WB |
| Anti-GADPH | ABclonal AC027 | Human GAPDH | 1:3000 for WB |
| Anti-FLAG | MBL M185-3L | FLAG | 1:5000 for WB |
| Anti-GFP | Absmart | GFP | 1:5000 for WB |
| Proteintech 50430-2-AP | GFP | 1 mg per 1 ml cell lysate for IP |