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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Microbiol. 2018 Oct 21;111(1):118–130. doi: 10.1111/mmi.14142

Figure 1. Full-length DnaA, DnaD and DnaB do not interact in a B2H assay.

Figure 1.

(A) A schematic of the helicase loading pathway in B. subtilis. Black arrows indicate potential protein-protein interactions while blue curved arrows indicate self-interactions. (B) B2H of T18- with T25-tagged DnaD (top) or DnaB (bottom) to demonstrate self-interactions mediated by the adaptor proteins. (C) B2H of T18-tagged DnaD co-expressed with either T25-tagged DnaA, wild type DnaB (DnaBWT), or the gain-of-function DnaB variant (DnaBS371P).