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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Microbiol. 2018 Oct 21;111(1):118–130. doi: 10.1111/mmi.14142

Figure 2. Mapping Interacting Domains Involved in DNA Replication Initiation.

Figure 2.

(A) Schematic of DnaA, DnaD, DnaB, DnaC, and DnaI divided into their individual domains. Amino acid boundaries of each protein fragment are indicated. WHD = Winged Helix Domain; CTD = C-terminal Domain; NTD = N-terminal Domain; ZBD = Zinc Binding Domain. (B) SDS-polyacrylamide gel showing purified domains from DnaD and DnaB. (C) B2H of T25-tagged DnaA domains co-expressed with T18-tagged full-length YabA (YabAFL), full-length SirA (SirAFL), or the domains from DnaD. (D) B2H of T25-tagged DnaB domains co-expressed with T18-tagged DnaD domains. (E) B2H of T25-tagged DnaI or DnaB domains co-expressed with T18-tagged DnaC domains. (F) B2H of T25-tagged DnaB domains co-expressed with T18-tagged DnaI domains.