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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Microbiol. 2018 Oct 21;111(1):118–130. doi: 10.1111/mmi.14142

Figure 3. The DnaDWHD Wing Forms a Binding Cleft for DnaA.

Figure 3.

(A) The crystal structure of DnaDWHD (PDB 2V79) is shown as a surface representation and colored according to sequence conservation as indicated in the legend (top). The boxed inset shows a semi-transparent surface with F51, I83 and E95 represented as sticks. (B) B2H of T18-tagged DnaDWHD variants co-expressed with either T25-tagged DnaADI, DnaBFL or DnaDWHD. (C) SDS-polyacrylamide gel stained with coomassie blue showing the glutaraldehyde crosslinking of wild type DnaDWHD (WT) or the F51A and I83A variants to reveal self-interactions. The various oligomeric forms are symbolized by dots at the right-hand side of the gel, with each dot representing one DnaDWHD protomer. The molecular weight marker is labeled on the left-hand side in kDa.