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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Microbiol. 2018 Oct 21;111(1):118–130. doi: 10.1111/mmi.14142

Figure 4. The DnaDWHD Binding Cleft is Essential.

Figure 4.

(A) dnaD23 strains ectopically expressing WT DnaD or its winged helix variants (F51A or I83A) were incubated at the permissive temperature (30°C) or the restrictive temperature (48°C). The dnaD23 parental strain was also included as a control (labeled “None” on the plates). (B) The same strains described in (A) were also incubated at the permissive temperature without (0%) or with (1%) xylose induction. Small colonies indicate a growth defect upon DnaD over-expression.