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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Microbiol. 2018 Nov 15;111(1):237–253. doi: 10.1111/mmi.14151

Figure 3. DdcA cannot complement loss of checkpoint recovery proteases.

Figure 3.

(A) Spot titer assay using B. subtilis strains WT (PY79), ΔddcA (PEB357), ΔddcA amyE∷Pxyl-ddcP (PEB836), and ΔddcA amyE∷Pxyl-ctpA (PEB837) spotted on the indicated media. (B) Spot titer assay using B. subtilis strains WT (PY79), ΔddcP ΔctpA (PEB555), ΔddcP, ΔctpA, amyE∷Pxyl-ddcA (PEB838), ΔddcP, ΔctpA, amyE∷Pxyl-ddcP (PEB557), ΔddcA ΔddcP ΔctpA (PEB639), ΔddcP, ΔctpA, ΔddcA, amyE∷Pxyl-ddcA (PEB840), ΔddcP, ΔctpA, ΔddcA, amyE∷Pxyl-ddcP (PEB839), and ΔddcP, ΔctpA, ΔddcA, amyE∷Pxyl-ctpA (PEB841) spotted on the indicated media. (C) Spot titer assay using B. subtilis strains WT (PY79), ΔddcP ΔctpA (PEB555), ΔddcP, ΔctpA, amyE∷Pxyl-ddcA (PEB838), ΔddcA ΔddcP ΔctpA (PEB639), ΔddcP, ΔctpA, and ΔddcA, amyE∷Pxyl-ddcA (PEB840) spotted on the indicated media.