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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Mol Microbiol. 2018 Oct 26;111(1):82–95. doi: 10.1111/mmi.14140

Figure 1. Killing of Y. pestis Δail by human serum is mediated by the alternative pathway of complement.

Figure 1.

~7.5 × 105 CFU of mid-log cultures of Y. pestis strains containing wild-type Ail, a chromosomal deletion of ail (Δail), or chromosomal ail recombinants were treated with 80% NHS, 80% HIS (Heat-inactivated serum), or 80% NHS-AP (NHS treated with 5mM EGTA and 10mM MgCl2 to inactivate CP/LP) for one hour at 37°C. Surviving bacteria were plated and enumerated by colony counting. Percent serum resistance was calculated as the number of surviving colonies in NHS/HIS or NHS-AP/HIS x 100 and is displayed on a logarithmic scale. Strains were tested a minimum of 3 times for each condition in separate experiments. Significance was determined using the two-way ANOVA with Tukey’s post hoc test. *, p-value < 0.05 when compared to the parental KIM5 wild-type (WT) strain in the same serum condition.