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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Hepatology. 2018 Dec 31;69(2):860–875. doi: 10.1002/hep.30229

Figure 1. Effects of local manipulation of gastric mTOR signaling by Cre adenovirus.

Figure 1.

Laparotomy was performed on 12 weeks old TSC1flox/flox mice to expose the left gastric artery, through which Cre adenovirus (1×109 pfu) was delivered locally to the stomach. GFP adenovirus (Ad-GFP) was used as control. All values are expressed as mean ±SEM. * indicates p<0.05 vs. Ad-GFP group, n=12 mice for each condition.

a. Schematic diagram of blood supply for stomach and location of left gastric artery for injection. Tracing of typan-blue injected through the left gastric artery at a dose of 50μl showed the feasibility of injection.

b. Knockdown of TSC1 and activation of mTOR signaling in gastric mucosa were validated by Western blotting.

c. Levels of ghrelin mRNA analyzed by RT-qPCR. Glyceraldehyde phosphate dehydrogenase (gapdh) was used as reference to normalize ghrelin expression.

d. Food intake and percentage of body weight change were recorded daily for 1 week.

e. One week after injection, hepatic lipid was extracted and assayed for triglyceride.