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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Hepatology. 2018 Dec 31;69(2):860–875. doi: 10.1002/hep.30229

Figure 6. Effects of ghrelin supplementation in TG mice.

Figure 6.

Wild-type littermates (WT) and TG mice were fed HFD for 12 weeks and administered acyl-ghrelin (11nmol/kg/day) for 2 weeks through a subcutaneous osmotic mini-pump. Levels of mRNAs were determined by RT-qPCR and normalized to β-actin. Fold changes were expressed as means±SEM. n = 10 for each group. *P<0.05 vs. WT (saline), # P<0.05 vs. TG (saline)).

a. Oil-red O staining.

b. Hepatic and circulating triglyceride content measured by colorimetric assay.

c. Hepatic mRNA levels of lipogenesis-, lipid transport- and β-oxidation-related genes.

d. Representative H&E staining of subcutaneous white adipose tissue (sWAT).

e. mRNAs levels of brown- or white- marker genes in sWAT.