Fig. 4.

Frequency distribution of the relative thiol oxidation of the identified cysteine-containing peptides inE. colicells during phagocytosis. E. coli cells were incubated with neutrophils for 2 h. Extracellular E. coli cells were separated from the neutrophils and the intracellular E. coli cells enriched thereafter. Both the extracellular (A) and the phagocytized (B)E. coli cells were analyzed using OxICAT. The relative thiol oxidation of 173 matched cysteine residues were visualized using frequency distribution analysis.