Fig. 6.

Differentially oxidized protein cysteine residues of phagocytizedE. coliand their functional classification. A. A volcano plot shows the difference of thiol oxidation, as determined by OxICAT, between E. coli cells, which experienced host neutrophil phagocytosis to extracellular bacteria. Log₁₀ of p-values from the Student´s t-distribution is plotted against the difference in thiol oxidation. The non-axial vertical line denotes an increase of 30% thiol oxidation while the non-axial horizontal line denotes a p-value of 0.01. Cysteine-containing peptides below these thresholds are shown as gray dots, peptides crossing the thresholds as black dots. B. All predicted proteins from E. coli K12 MG1655 (ecogene.org,16.05.2018) and their respective cellular functions. C. 76 Significantly thiol-oxidized proteins (difference > 30%; p > 0.01) and their respective cellular functions. Proteins involved in stress response, cell detoxification, carbohydrate and protein metabolism are overrepresented in our dataset.