Fig. 3.

NASH diet induced liver oxidative stress was exaggerated in adropin-KO mice. Adropin-KO mice and the wild type (WT) littermate were fed with MCD or WD for 4 or 16 weeks. (A) DHE and TUNEL staining of liver sections (magnification, ×200), scale bar: 200 µm. (B) Quantitative analysis of DHE staining. (C) Quantitative analysis of TUNEL staining. (D) The liver MDA contents. (E) The liver GSH levels. (F-G) Cleaved caspase-3 expression of total liver lysates. (B, G) WT control group was set as 1. The data are expressed as the mean ± SD, n = 6, * P < 0.05 versus WT control.