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. 2019 Jan 25;5(1):e01167. doi: 10.1016/j.heliyon.2019.e01167

Fig. 1.

Fig. 1

The biogenesis of intronic miRNA miR-302. Encoded in the intronic region of the La Ribonucleoprotein domain family member 7 gene (LARP7 or PIP7S), miR-302 is a native intronic miRNA. First, transcribed within the intron of LARP7 gene transcripts by type-II RNA polymerases, and then cellular spliceosomes splice the intron out of LARP7 gene transcripts to form primary miRNA precursors, pri-miRNAs (pri-miR-302). After that, Drosha-like endoribonucleases process the pri-miRNAs into miRNA precursors (pre-miRNAs) with a single hairpin like structure. Then, Exportin-5 exports the pre-miRNAs out of the nucleus into the cytoplasm allowing Dicer-like RnaseIII endoribonucleases to further process and form 21–23nt long mature miRNAs, such as miR-302s. Lastly, following assembly into RNA-induced silencing complexes (RISC) with Argonaute proteins, the mature miR-302s carry out their specific gene silencing functions.