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Cellular-based bioassay for L. lactis-secreted bioactive IL-17. (A) IL-6 secretion by 3T3 L1 cells after exposure to supernatant samples of recombinant L. lactis. (B) Quantification of IL-17 production by recombinant lactococci by ELISA. Data are represented as mean ± SEM of two independent in vitro assays. GM17 was used a control for supernatant conditions. Stress was induced with NaCl 2.5%. Data were treated by ANOVA (Turkey post-test).
