Figure 4.

Learning and long-term memory deficits of Chd1^Δ2/Δ2 mice in the Barnes Maze (BM) test. (A) Schematic representation of BM experimental set-up. Animals were trained during 4 days with three training trials on each day to find the target hole (black circle). On day 6, all holes were closed and animals were tested for short-term memory. On day 13, animals were tested for long-term memory. (B,C) Learning was monitored by recording the time required to find the target hole (latency; B) and by determining the number of errors before finding the target hole (C) during each training trial. Statistical analysis was performed by two-way repeated measures ANOVA (n = 8/group). (D,E) Short term memory (D) and long-term memory (E) were assessed by recording the time spent in each quadrant in the test trial at day 6 (D) and 13 (E). Statistical analysis in (D,E) was performed by one-way ANOVA for each genotype (n = 8 per group). q1–4, quadrants 1–4; q1 is the target quadrant. Means ± SEM are shown. Statistical significance threshold was set to p < 0.05 (*p < 0.05, **p < 0.01, ***p < 0.001, ns, non-significant).