Figure 5.

Dysregulation of immediate early genes (IEG) in the hippocampus of Chd1^Δ2/Δ2 animals. (A) Reverse transcription real-time PCR was performed on RNA from dorsal hippocampi (dHC) isolated from Chd1^Δ2/Δ2 and Chd1^flox/flox mice kept in their home cage (“naïve”; n = 4/4), mice from both groups that were habituated to the test arena but not trained or tested (“handled”; n = 3–4/group), animals that were sacrificed 30 min after long-term OLM training (“OLM trained”; n = 4/4) or 30 min after long-term OLM testing (“Post LT OLM”; Chd1^flox/flox n = 9, Chd1^Δ2/Δ2 n = 8). (B) Expression of Egr1 and Arc correlates with the performance (discrimination index) of the respective Chd1^flox/flox mice in the OLM test, while no such correlation was detected for Chd1^Δ2/Δ2 animals. Correlation coefficients (r) and p values are shown in the figure legends. Statistical analysis was performed by pairwise t-test with Holm-Sidak correction for multiple testing for (A) and using the built-in correlation analysis tool of GraphPad Prism 7 for (B). p < 0.05 was considered significant (*p < 0.05).