Skip to main content
. 2019 Jan 23;12:3. doi: 10.3389/fnmol.2019.00003

Table 1.

Sequences of primers used in reverse transcription quantitative PCR (RT-qPCR) and ChIP-qPCR.

RT-qPCR Forward primer Reverse primer
Bdnf 5′CGGACCCATGGGACTCTGGA 5′GTTGGGCCGAACCTTCTGGT
Arc 5′CGCAGAAGCAGGGTGAACCA 5′TCCTCCTCCTCAGCGTCCAC
Egr1 5′GCGCCCACCTTTCCTACTCC 5′CCAGGCTCAGGTCTCCCTGT
cFos 5′AGCAACGTGGAGCTGAAGGC 5′TGTGCAGAGGCTCCCAGTCT
Grin2A 5′AACTACAAGGCCGGGAGGGA 5′ACTGGAGCAGAGCGAGGTCA
TBP 5′TAATCCCAAGCGATTTGCTGC 5′TTCACTCTTGGCTCCTGTGC
ChIP qPCR Forward primer Reverse primer
amplicon centered at position
−180 (Egr1) 5′GCTTTCCAGGAGCCTGAGC 5′AGCCCTCCCATCCAAGAGT
−126 (Egr1) 5′GTGCCCACCACTCTTGGAT 5′GCAGGAAGCCCTAATATGGA
−69 (Egr1) 5′GCCGGTCCTTCCATATTAGG 5′TCAAGGGTCTGGAACAGCAC
38 (Egr1) 5′CGAGAGATCCCAGCGCGCAG 5′TCTTGCGGCGGCGGAAGCTG
171 (Egr1) 5′GGGGCCCACCTACACTCC 5′GTTGGCCGGGTTACATGC
2204 (Egr1) 5′CAGAAGCCCTTCCAGTGTCG 5′GATGGGTAGGAGGTAGCCAC
2918 (Egr1) 5′CCCTTCAGCGCTAGACCATC 5′GCTGTACAAAGATGCAGGGC
β-actin 5′ACGCCATCCTGCGTCTGGAC 5′ATGACCTGGCCGTCAGGC