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. 2019 Jan 23;9:3325. doi: 10.3389/fmicb.2018.03325

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Copyright © 2019 Zhang, Chen, He, Huang, Li, Lai, Zhan, Huang, Liu, Wu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Asprellcosides B attenuates influenza A virus mRNA transcription in MDCK cells. (A,B) The inhibitory effect of Asprellcosides B on the expression of influenza A/FM/1/47(H1N1) HA mRNA and NA mRNA were detected by quantitative real-time PCR at 48 h post-infection. ^∗∗p < 0.01, ^∗p < 0.05. Assessed the antiviral activity of other influenza virus. (C) The antiviral activity of H1N1 PR8, H9N2, H3N2, and HSV virus were assessed through CPE inhibition by using WST-8 assay, and (D) the antiviral activity of ZIKA and DEVN virus was determined by quantitative real-time PCR. (E) The plaque was fixed and stained at 48 h post infection, and the experimental method was detailed in the Section “Materials and Methods.”