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. 2019 Jan 23;9:3038. doi: 10.3389/fimmu.2018.03038

Figure 3.

Figure 3

Germinal center responses and TFH cell profile of adoptively transferred CD4 T cells following challenge a BALB/c heart allograft. (A) Representative confocal immunofluorescence photomicrograph of a splenic B cell follicle at d14 (left; scale bar−50 μm) and d50 (right; scale bar−250 μm) from reconstituted BL/6 Tcrbd−/− recipients of BALB/c heart grafts stained with antibodies against B220 (B cells, blue), GL7 (GC B cells, green), and CD4 (T cells, red), demonstrating poorly defined GCs at d14 but by d50 a typical secondary follicle, with TFH cells present within the GC (inset), readily evident. (B) Histogram of secondary follicles expressed as percentage of total follicles within d50 spleens of BL/6 Tcrbd−/− recipients either non-reconstituted (naïve) or adoptively transferred with 1 × 103 WT or Sh2d1a−/− (SAP−/−) TCR75 CD4 T cells. *P = 0.01, Mann-Whitney test. (C) ELISPOT assay of splenic and bone-marrow anti-Kd IgG antibody secreting cells (ASCs) 50 days after transplantation. Numbers of bone marrow ASCs in recipients reconstituted with SAP−/− TCR75 CD4 T cells were not above background. Data represents mean ± S.E.M of a minimum of 5 animals/group, with each dot representing the biological replicate in a distinct animal; *1P = 0.02, #2P = 0.15, *3P = 0.02, Mann–Whitney test.