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. 2019 Jan 29;10(2):92. doi: 10.1038/s41419-019-1308-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a A time-course of TGF-β₁-induced TTC3 expression in BEAS-2B cells and NHLFs. BEAS-2B cells and NHLFs were treated with 10 ng/ml TGF-β₁ and harvested at the indicated time points. b Inhibition of TGF-β₁-induced elevation of total and phosphorylated forms of SMAD2 and SMAD3 in BEAS-2B cells by SMURF2 overexpression. After transfection of SMURF2/Myc, BEAS-2B cells were treated with 10 ng/ml TGF-β₁ for 1 d. c SMAD2/3-mediated TTC3 induction in TGF-β₁-treated BEAS-2B cells. BEAS-2B cells were transfected with SMAD2 and SMAD3 siRNAs and treated with 10 ng/ml TGF-β₁ for 1 d