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. 2019 Jan 29;9:837. doi: 10.1038/s41598-018-37139-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Prx1 marks a population of cells in mouse kidney. (A) Microscopic images of kidney sections of Prx1-Cre; tdTomato mice. (B) Immunofluorescent staining on Prx1-Cre; tdTomato mouse kidney sections revealed that Prx1 linage renal cells were negative for the tested markers of different renal cell types. (C) DT was injected into Prx1-Cre; iDTR mice daily for different periods of time as indicated to deplete Prx1 lineage cells. Moue kidneys were sectioned and stained with H-E. (D) Quantitative analysis of tubular cell numbers. N = 3. *P < 0.05; **P < 0.001 when compared to the control mice.