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. 2019 Jan 29;9:891. doi: 10.1038/s41598-018-37385-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of GPNA on BEAS-2B. (a) Western blot analysis of GGT and ASCT2 expression in A549 and BEAS-2B cells. Full-length blots are presented in Supplementary Fig. S6. (b,c) BEAS-2B cells were incubated for 48 hrs with 250 μM GPNA. Where indicated, 20 mU/ml of purified human GGT (hGGT) and 20 μM GGsToP were added to the incubation mixture. The “GGT tr.” sample corresponds to BEAS-2B cells stably transfected with human GGT (see 2.2 section in Material and Methods for further details). (b) Cell viability with the resazurin method; (c) Apoptosis index with Hoechst staining. Data are expressed as means ± s.d. of six values and were analyzed by one-way ANOVA with Newman–Keuls test for multiple comparisons. (*)p < 0.0001 as compared to “GPNA”.