Table 3.
Apparent kcat for Various GUS Enzymes with DCF-G.
| Loop Class | GUS | Optimal pH | Apparent kcat (s−1) |
|---|---|---|---|
| L1 | E. coli | 7.4 | 30.7 ± 0.7 |
| L1 | E. eligens | 6.5 | 138 ± 2 |
| L1 | S. agalactiae | 6.5 | 97 ± 5 |
| L1 | C. perfringens | 6.5 | 61.5 ± 0.6 |
| L1 | F. prausnitzii | 6.0 | 32 ± 3 |
| L1 | L. rhamnosus | 4.5 | 10 ± 1 |
| L1 | R. gnavus | 6.5 | 24.0 ± 0.5 |
| mL1 | B. fragilis | 5.0 | 15.1 ± 0.5 |
| L2 | B. uniformis | 5.5 | 10 ± 1 |
| mL2 | P. merdae | 5.5 | 0.034 ± 0.001 |
| mL1,2 | B. ovatus | 5.0 | 1.64 ± 0.04 |
| NL | B. dorei | 6.0 | 9.8 ± 0.6 |
Data are presented as the average of 3 biological replicates and errors are SEM.